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cell signal 2006  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signal 2006
    Cell Signal 2006, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signal+2006/pm39514408-457-3-3?v=Cell+Signaling+Technology+Inc
    Average 93 stars, based on 7 article reviews
    cell signal 2006 - by Bioz Stars, 2026-07
    93/100 stars

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    Figure 4. Non-canonical Rag GTPase Regulation in Steady-State ESCs (A and B) Tfe3 localization (A) and retention of self-renewal after 3 days of 2i withdrawal (B) of the indicated genotypes. Average and SD of 2 biological replicates are shown. Scale bar, 20 mm. (C) mTORC1 activity in ESCs in response to amino acids. Arrows mark fast-migrating (unphosphorylated) and slow-migrating (phosphorylated) 4EBP1. (D) Retention of self-renewal after 3 days of 2i withdrawal in the indicated cell lines in the absence or presence of 20 nM rapamycin. Average and SD of 2 biological replicates are shown. (E and G) Tfe3 subcellular localization in the indicated ESCs with or without 20 nM rapamycin (E) and <t>Tsc2</t> KO.1 ESCs expressing the indicated Rag constructs (G). Scale bar, 20 mm. (F) Retention of self-renewal relative to empty vector-transfected cells in Tsc2KO.1 cells expressing the indicated transgenes after 3 days of 2i withdrawal. Average and SD of 2 biological replicates are shown.
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    Figure 4. Non-canonical Rag GTPase Regulation in Steady-State ESCs (A and B) Tfe3 localization (A) and retention of self-renewal after 3 days of 2i withdrawal (B) of the indicated genotypes. Average and SD of 2 biological replicates are shown. Scale bar, 20 mm. (C) mTORC1 activity in ESCs in response to amino acids. Arrows mark fast-migrating (unphosphorylated) and slow-migrating (phosphorylated) 4EBP1. (D) Retention of self-renewal after 3 days of 2i withdrawal in the indicated cell lines in the absence or presence of 20 nM rapamycin. Average and SD of 2 biological replicates are shown. (E and G) Tfe3 subcellular localization in the indicated ESCs with or without 20 nM rapamycin (E) and Tsc2 KO.1 ESCs expressing the indicated Rag constructs (G). Scale bar, 20 mm. (F) Retention of self-renewal relative to empty vector-transfected cells in Tsc2KO.1 cells expressing the indicated transgenes after 3 days of 2i withdrawal. Average and SD of 2 biological replicates are shown.

    Journal: Cell stem cell

    Article Title: Lysosomal Signaling Licenses Embryonic Stem Cell Differentiation via Inactivation of Tfe3.

    doi: 10.1016/j.stem.2018.11.021

    Figure Lengend Snippet: Figure 4. Non-canonical Rag GTPase Regulation in Steady-State ESCs (A and B) Tfe3 localization (A) and retention of self-renewal after 3 days of 2i withdrawal (B) of the indicated genotypes. Average and SD of 2 biological replicates are shown. Scale bar, 20 mm. (C) mTORC1 activity in ESCs in response to amino acids. Arrows mark fast-migrating (unphosphorylated) and slow-migrating (phosphorylated) 4EBP1. (D) Retention of self-renewal after 3 days of 2i withdrawal in the indicated cell lines in the absence or presence of 20 nM rapamycin. Average and SD of 2 biological replicates are shown. (E and G) Tfe3 subcellular localization in the indicated ESCs with or without 20 nM rapamycin (E) and Tsc2 KO.1 ESCs expressing the indicated Rag constructs (G). Scale bar, 20 mm. (F) Retention of self-renewal relative to empty vector-transfected cells in Tsc2KO.1 cells expressing the indicated transgenes after 3 days of 2i withdrawal. Average and SD of 2 biological replicates are shown.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FLAG Sigma Cat#F1804; RRID:AB_262044 LAMP1 DSHB Cat#1D4B; RRID:AB_2134500 Lamtor1 Cell Signaling Cat#8975S; RRID:AB_10860252 RagB Cell Signaling Cat#8150S; RRID:AB_11178806 RagC Cell Signaling Cat#9480S; RRID:AB_10614716 Tfe3 Sigma Cat#HPA023881; RRID:AB_1857931 GAPDH Sigma Cat#G8795; RRID:AB_1078991 4EBP1 Cell Signaling Cat#9452S; RRID:AB_331692 S6 Cell Signaling Cat#2217; RRID:AB_331355 S6K1 Cell Signaling Cat#9202S; RRID:AB_331676 pS6 Cell Signaling Cat#2211; RRID:AB_331679 pS6K1 Cell Signaling Cat#9205; RRID:AB_330944 Flcn Baba et al., 2006 N/A Tsc2 Cell Signaling Cat#4308S; RRID:AB_10547134 Biological Samples Skin and blood samples from 26 affected patients and their parents.

    Techniques: Activity Assay, Expressing, Construct, Plasmid Preparation, Transfection